FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5477781
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG315870.5766386060340858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG97500.08.4939071
GCGGTTT12100.00369891386.5466871
GGCAATT33153.7107384E-106.0825931
GTTTACC28551.3495628E-75.80144261
CTAGTTA14950.0052056045.7739658
GGAAAAT98800.05.5395051
GTTGGAT33158.493407E-85.43088631
GGAAACT59250.05.22630021
GTGAAAT59300.05.1004541
GTTAAGT25551.3227185E-45.07336241
TGGGGGG173550.05.0623191
GTTTGAT45651.195076E-95.0480481
GCAAAAT68500.05.04620551
GCTAAGT20100.004511945.01586961
GCCAAAT46001.4661055E-95.00963931
Produced by FastQC (version 0.11.5)