FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9558817
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132900.13903394112472287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAA21802.1874621E-76.6208551
CTTAAAC49903.6379788E-125.35108381
GCGAGAT26153.299005E-55.2435151
GCTAAAT46151.5133992E-95.0040181
Produced by FastQC (version 0.11.5)