FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8280390
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC15150.025.2811071
CCGATCT20100.019.7741072
CGATCTA10509.085237E-68.9471963
CGATCTC32659.422365E-95.75470453
CCAACCG27700.00223391535.3064017145
ACGGGCG29200.0043163395.0338125145
GTTAGAA53302.3646862E-115.01657631
Produced by FastQC (version 0.11.5)