FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9060216
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAC26600.07.8726041
GCCTAAC26451.8189894E-127.6442411
ATTTCGC16104.6946225E-67.17622661
GCTTTAC51450.07.01756761
TGGGGGG197650.06.61277531
GCTTTAA69150.06.26557731
GTTTAAC40253.6379788E-115.74098061
GCTTCAC96750.05.6723711
GCTTTAT70400.05.5388951
GATTTAT71450.05.35643241
GTTTCAC78800.05.31500631
GCTTCGT31358.3402483E-75.2977611
TGCACTC118850.05.225182
GGCTAAC27851.6762635E-55.18569331
GCACTCC116750.05.13361363
AGGGGGG99200.05.0955181
GTTTTTT188600.05.05398851
GTTTACA67350.05.03920751
Produced by FastQC (version 0.11.5)