FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7897284
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257130.32559295069038924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG127350.08.0453271
GCGTAAA8200.0058008017.919231
GCGAAAT15252.1099477E-67.5701491
CGTAAAA16200.00195123285.78524832
GCCGAAT15250.0062731365.6776121
GGGGGTT97600.05.5392034
Produced by FastQC (version 0.11.5)