FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8185117
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAAAT65950.06.3526261
GTTTTTT166950.06.2304031
GTTTAAC37251.6552804E-105.8174821
GTTCGGA15100.00555378575.74042841
GTTAAGG34154.3983164E-95.71101241
GGGGGGG593050.05.4941664145
GCGGAAT15850.0094816035.46879961
GGCAAAT62000.05.35927721
GTTCAAT46405.820766E-115.2929891
CTCAAAT72650.05.26963231
TATCTAG26053.08472E-55.26475245
GTTAGAC19400.00279301475.21274331
GTGAAAT96200.05.18100551
GTAAAAT96350.05.172941
GTTGAAT57800.05.1238461
Produced by FastQC (version 0.11.5)