FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9236453
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174970.18943419080896098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG73950.017.7816945
CTATACT46950.016.1008054
ATACTGT92950.015.5141946
TCTATAC48350.015.5033843
TGACGCG22300.015.133124145
ACTGATC54800.015.0706518
GTACTAG23150.014.8322821
CTAGGAC37450.014.4181023
GTATAGA56500.014.1803621
GTGTAGG56200.014.1429141
TAGACAG82850.014.0313535
TATACAG68500.014.0032015
TACACTG75850.013.7350185
CCTACAC50650.013.2943593
CTACACT58950.013.2543234
GTATAGG33250.013.0041651
GTCTTAC43650.012.9649531
CCTAGAC41250.012.935893
GTGTTAG49300.012.76891
GTATATA133750.012.6459971
Produced by FastQC (version 0.11.5)