FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16394978
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC116450.021.5557638
TATACTG136900.020.2341165
TACACTG137600.018.731175
CTATACT83950.018.199474
CTACACT101550.017.4001644
CCTATAC55100.016.7576663
GTCCTAG74700.016.4534551
TGCACTG229550.015.4820385
TAGACAG155300.015.4414245
CCTACAC97350.015.35312753
TCTATAC85400.015.3235243
ATACTGT173500.015.0848766
GTATAGG62550.014.9760681
CTGATCA159300.014.5068999
TAGACTG93050.014.1351515
GTGTTAG97050.014.0334461
GTGTAGG100500.013.882231
CTAGCAC84350.013.8604673
TATACAG134750.013.8032365
GTCTAGA81400.013.7116861
Produced by FastQC (version 0.11.5)