FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16394978
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN204010.12443444571868288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC114500.018.714968
TATACTG137200.018.3252165
TACACTG138900.017.6710975
CTAGGAC74050.017.3794773
CTACACT99950.016.4598644
TAGACTG97300.015.8175485
CTAGACT69250.015.6143974
CTATACT87650.015.5152694
GTATAGG64000.015.3567131
GTCCTAG73750.015.1273831
GTATTAG84000.015.0207231
ATACTGT171700.014.9527336
TAGACAG155150.014.9223645
TAGGACT81300.014.76880554
GTGTAGG109000.014.7436651
TCTATAC85000.014.6723253
CTTACAC95450.014.5949763
CCTACAC97500.014.4922243
GTGTTAG103450.014.3791781
GTATACG20100.014.2065611
Produced by FastQC (version 0.11.5)