FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682399
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG78000.017.0720845
CTACACT57400.016.7675934
ACTGATC62050.016.6754518
GTATAGG34200.016.5783121
GTATTAG49000.016.5491981
GTCCTAG43700.016.4442081
CTATACT51750.016.4327094
TATACTG76100.015.8524445
TCTATAC53200.015.4891713
CCTACAC54050.015.0016573
GTATAGA52800.014.3592541
GTGTAGA61750.014.1998131
TAGACTG55000.014.1432455
ATACTGT96900.013.94646
GTCTAGA49300.013.907671
CCTATAC35650.013.8685653
CTTACAC54400.013.8145173
GTGTAGG57600.013.6204811
GTCCTAC38900.013.5583971
GTCTTAG45850.013.2286791
Produced by FastQC (version 0.11.5)