FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9462500
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAAAT48550.06.5356061
GCCAAAT72500.05.9680941
AAAATCG25706.864557E-75.8878873
GCGAAAT19354.5774542E-45.59026861
GTTAAAT77350.05.40741971
GGGGGGG778650.05.2968173145
AAATCGG19450.00297412325.1866434
GTTTAGA50252.1827873E-115.16640951
GCTAACT32151.4108264E-65.15904431
GTTAAGT38206.155096E-85.0970961
GGAAAAT158850.05.0845581
AAATCGT20000.0042101675.044014
Produced by FastQC (version 0.11.5)