FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14492287
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN169220.11676555950071925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG138850.020.0196935
CCTATAC49750.017.0989863
ATACTGT171350.016.9656266
TACACTG129550.016.9069615
ACTGATC95550.016.47468
CTATACT82550.016.2277814
TCTATAC80100.015.3866833
GTATAGG57050.015.2802121
GTCCTAG66900.015.1353511
CTACACT92850.015.0768494
GTATTAG75750.014.7834411
CTAGGAC64450.014.550063
GTGTAGG93750.014.5199941
CCTACAC82250.014.4958543
TATACAG120750.014.20064455
GTATATA237550.014.0577551
GTATACG19100.014.0432721
CTAGTAC39350.013.9581813
TGCACTG182950.013.7660915
GTATAGA90550.013.4779741
Produced by FastQC (version 0.11.5)