FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6792464
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149440.22000852709708876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG55200.017.4814285
GTCCTAG28350.017.0325281
GTATTAG33600.015.9041231
GTATAGG23900.015.6243161
GTATAGA40800.015.3067321
ACTGATC41450.015.2104578
GTATAAG34100.014.7268921
CTTACAC36800.014.6846163
TACACTG56100.014.6781665
CCTACAC35500.014.4974873
CTATACT34400.014.0254864
TAGACAG60750.013.8723395
CCTATAC23700.013.8437243
ATACTGT71150.013.2008716
GTATACG7854.2382453E-1013.1226411
CTAGGAC28850.012.9334173
GTATATA98200.012.8503671
GTCTAGA35850.012.7508781
GTCTTAC30300.012.7491011
TAGGACT32800.012.7483694
Produced by FastQC (version 0.11.5)