FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6880127
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG131350.010.1285191
AGGGGGG63500.08.7200971
GTTTAAC35151.8189894E-126.5467981
GTCAATT38501.8189894E-126.1639271
GTCTAAT24803.6614983E-76.08936361
AGGTCGT20350.0082503526.060847145
GTTTAGG32301.3133103E-96.01126051
GCTTAGT23054.2118863E-65.927711
GGTTAAT34107.6579454E-105.9048371
GTCACTT64900.05.87266831
GGAAAAT131750.05.84033061
GATCGCT15400.00725172555.603571
GTTTTTT163900.05.57223031
GGAAATT86450.05.49013761
GTAAAAT90400.05.4888951
GTTCAGT56550.05.4681461
GTTTATT88750.05.42888641
GTAGAAT43202.8012437E-105.32685041
GGAATTT89200.05.3208791
GTTAAAC32453.2046046E-75.3186431
Produced by FastQC (version 0.11.5)