FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14667742
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184110.12552034253124986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCGG36358.160683E-65.700455145
GCGCGAT26751.5494334E-65.63502171
CGCGATC23804.598062E-55.42513942
AAATCGC29200.00268746215.228842145
Produced by FastQC (version 0.11.5)