FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9352320
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG134590.14391081571203723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCG9850.008170318.839621145
TCGAGCG15104.8564377E-47.928583145
GTGCGAC18150.00341831456.596232145
CGTCGTG29458.074647E-55.913091145
TACGGGC18300.008581285.1023439
Produced by FastQC (version 0.11.5)