FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3720968
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71150.19121368418110557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAAT25251.02136255E-86.58170131
GTGGATT28853.7627926E-45.6713805145
TTGTAGT27200.00109509685.614389145
AGGGGGG75950.05.6130121
AGTAAGT33655.255459E-45.1865463145
Produced by FastQC (version 0.11.5)