FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9629549
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156170.16217789638954017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGACG23759.714607E-46.1318674145
CTTTACG15850.00159302235.88438036
TTGGGCG32603.6784673E-55.8417687145
GTTAAGT45405.456968E-125.5415861
GTTCGAT17050.00370704785.480761
GTCAAAT74350.05.3174521
GTAAATT96600.05.2832731
AGGGGGG229750.05.16238361
GGAAAAT189550.05.0057831
Produced by FastQC (version 0.11.5)