FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15497635
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG145400.019.6636565
CTATACT86700.017.7155444
ACTGATC106650.017.6377898
TACACTG129350.017.2716185
TCTATAC89700.017.0489233
ATACTGT176550.016.3825596
GTGTAGG90900.016.0944121
CCTACAC85600.015.4575593
TATACAG131950.015.3187875
GTATAGG57050.014.6869351
TGCACTG211000.014.6216275
CTAGGAC73250.014.3420433
TAGACAG156100.014.3118765
GTCCTAG68000.014.2777651
CCTATAC56900.014.0226653
CTTACAC92850.013.9641443
GTATTAG78100.013.878831
CTACACT97450.013.7825994
ACTGTAC146300.013.4028228
TAGGACT83500.013.2981614
Produced by FastQC (version 0.11.5)