FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4695049
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTAGG21351.0584063E-66.4264795
GTTCAAA34358.221832E-105.88776541
GTCAAAT32357.719791E-95.8052141
CCTAAAT22801.2608826E-45.38558961
GTGAAAT48602.2009772E-105.0531461
TTTAGCC24353.3930322E-45.04029947
Produced by FastQC (version 0.11.5)