FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19287837
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT644450.015.782867145
CCGTATC578750.015.670533145
CGTATCA510650.015.446249145
CGCCGTA716750.015.445031145
GCGTCGG82300.014.886903145
GTATCAT519050.014.134771145
TCGCCGT810300.013.8945055145
GTCGCCG915000.013.485154145
GGTCGCC1016700.013.434008145
ATCATTA480700.013.196276145
TGGTCGC1100550.012.739846145
TATCATT538350.012.604587145
GTGGTCG1218250.012.473042145
TCATTAA487300.011.247159145
TCGGTGG1608850.011.00843145
CGGTGGT1479850.010.851093145
CTCGGTG1756300.010.761178145
GGTGGTC1446500.010.690301145
TCTCGGT1843150.010.29344145
CATTAAA487950.010.132732145
Produced by FastQC (version 0.11.5)