FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22576997
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC501400.016.015924145
TATGCCG401700.015.664724145
TCGTATG560650.015.421168145
CTCGTAT612850.014.792954145
ATGCCGT387050.014.293252145
TGCCGTC382650.013.908823145
ATCTCGT740800.013.733465145
GTATGCC498350.013.236973145
TCTCGTA661300.013.139761145
CCGTCTT322700.012.588346145
GATCTCG861550.012.287729145
GCCGTCT339650.012.258603145
TGATCTC1089100.010.657873145
CTGATCT1146050.010.532629145
CGTCTTC332050.010.511104145
TCTGATC1175850.010.49355145
GATCTGA1462450.09.972022145
ATCTGAT1299050.09.799361145
AGATCTG1636050.09.772535145
CAGATCT1746050.09.053194145
Produced by FastQC (version 0.11.5)