FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18609025
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT467800.016.28769145
CGTATCA371500.015.8458145
CCGTATC417950.015.663197145
CGCCGTA520050.015.055514145
GTATCAT393250.014.84035145
GTCGCCG680200.014.367137145
TCGCCGT596050.014.023732145
GGTCGCC759600.013.743411145
GCGTCGG73750.013.663746145
TGGTCGC823500.013.319634145
GTGGTCG927500.013.311214145
TCGGTGG1249850.012.018459145
ATCATTA372700.011.904441145
CGGTGGT1149300.011.789432145
TATCATT415200.011.471626145
GGTGGTC1141200.011.2696085145
CTCGGTG1377650.011.240337145
TCTCGGT1444500.010.810484145
TCATTAA381450.010.719104145
GATCTCG1695050.010.645335145
Produced by FastQC (version 0.11.5)