FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20681401
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG92750.017.899313145
CGTATCA568250.016.904068145
CCGTATC643250.015.812213145
GCCGTAT716400.015.371527145
CGCCGTA791900.014.665842145
TCGCCGT901400.014.452578145
GTATCAT572150.014.419399145
GTCGCCG1021400.014.330299145
TGGTCGC1238150.013.51965145
GGTCGCC1138200.013.496687145
TATCATT584350.013.001786145
GTGGTCG1375550.012.685701145
ATCATTA522450.012.571823145
TCATTAA520150.011.986287145
TCGGTGG1846650.011.828491145
CGGTGGT1688100.011.76704145
GGTGGTC1641000.011.428852145
CTCGGTG2019150.011.23804145
TCTCGGT2138200.011.073442145
ATCTCGG2338500.010.531081145
Produced by FastQC (version 0.11.5)