FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21396637
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAA57100.022.344149145
TATGCCG604650.016.077257145
ATGCCGT560900.015.87085145
CGTATGC761900.015.25186145
GCCGTCT473550.015.170331145
GTATGCC725500.014.618209145
TCGTATG845650.014.5728855145
CTCGTAT920150.014.535334145
TCTCGTA1002250.014.176442145
TGCCGTC529000.013.922758145
CCGTCTT430750.013.917701145
ATCTCGT1114950.013.445668145
CGTCTTC424400.013.186489145
AATCTCG1224000.013.130205145
TAATCTC1428250.011.886935145
GCGTTAA1968800.011.605714145
GTTAATC1606700.011.491625145
TTAATCT1564250.011.446641145
CGTTAAT1741700.011.312624145
AGCGTTA2138950.010.94346145
Produced by FastQC (version 0.11.5)