FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18009372
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG69200.016.86681145
CGTATCA321900.016.102682145
GCCGTAT401350.015.805126145
CGCCGTA445950.015.68752145
CCGTATC363050.015.2160425145
TCGCCGT514250.014.351149145
TGGTCGC707300.014.072783145
GTCGCCG588900.013.652215145
GTATCAT350250.013.536698145
GGTCGCC652950.013.256766145
GTGGTCG793650.013.171929145
CGGTGGT986700.011.82916145
TCGGTGG1075650.011.76756145
TATCATT380300.011.304244145
CTCGGTG1188450.011.163062145
TCTCGGT1242050.011.113249145
GGTGGTC985200.011.089246145
GATCTCG1463150.011.019429145
ATCATTA338100.010.635299145
ATCTCGG1362150.010.633684145
Produced by FastQC (version 0.11.5)