FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19572009
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG437300.016.112158145
ATGCCGT407350.016.015545145
TCGTATG611750.015.333003145
CGTATGC547500.015.225819145
CTCGTAT669550.014.366627145
GTATGCC537100.014.346469145
TGCCGTC392700.014.176442145
GCCGTCT348550.014.038012145
CCGTCTT317100.013.670105145
TCTCGTA719650.013.46719145
ATCTCGT804300.013.464786145
CATCTCG922150.012.852366145
CGTCTTC322000.012.561608145
GATCATC1244050.010.995145145
ATCATCT1254300.010.622115145
GAGATCA1623500.010.474726145
TCATCTC1199550.010.309262145
AGATCAT1402950.010.049498145
ACAAGAG2179200.09.832733145
CAAGAGA2130800.09.294049145
Produced by FastQC (version 0.11.5)