FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19572009
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA389550.016.826365145
GCGTCGG74100.015.949771145
CCGTATC440800.015.791185145
GCCGTAT489400.015.763866145
CGCCGTA542100.015.074037145
TCGCCGT621700.014.88178145
GTATCAT412150.014.197219145
GTCGCCG707750.013.902247145
TGGTCGC852700.013.5202875145
GGTCGCC785700.013.270497145
GTGGTCG955350.013.008685145
CGGTGGT1170300.011.883284145
TCGGTGG1278000.011.8463545145
CTCGGTG1417150.011.256204145
TCTCGGT1490900.011.2149105145
TATCATT443150.011.060659145
ATCTCGG1634700.011.0312145
GGTGGTC1173600.010.991094145
TCATTAA407750.010.936194145
ATCATTA400050.010.892943145
Produced by FastQC (version 0.11.5)