FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21038565
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC562450.016.034357145
CGTATCA496000.015.873154145
GCGTCGG97800.015.789043145
GCCGTAT623200.015.355415145
CGCCGTA692900.015.24418145
TCGCCGT796100.015.153074145
GGTCGCC1017800.014.003486145
GTCGCCG911550.013.941725145
GTATCAT510200.013.726247145
TGGTCGC1100000.013.596328145
GTGGTCG1226650.013.12041145
ATCATTA471750.012.755023145
CGGTGGT1509000.011.876113145
TATCATT527100.011.855758145
TCGGTGG1657600.011.847981145
CTCGGTG1821200.011.651455145
GGTGGTC1477500.011.398213145
TCTCGGT1927350.011.363319145
ATCTCGG2111900.011.063743145
TCATTAA477750.010.75872145
Produced by FastQC (version 0.11.5)