FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21516170
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT488000.015.819853145
CGTATGC656000.015.768589145
TCGTATG736550.015.608947145
TATGCCG522100.015.480816145
GTATGCC633650.014.620227145
CTCGTAT816150.014.37969145
ATCTCGT991250.014.355218145
CCGTCTT388100.014.176558145
TGCCGTC465600.014.058773145
GCCGTCT416650.014.04025145
TCTCGTA882750.013.475458145
CGTCTTC381900.012.565536145
CATCTCG1121300.012.367042145
CTCATCT1595500.010.699581145
TCATCTC1460950.010.682708145
ATCCTCA1987050.010.469978145
CCTCATC1714950.09.958559145
TCCTCAT1842300.09.8407145
ACCCATC2515200.09.78453145
CATCCTC2245800.09.70911145
Produced by FastQC (version 0.11.5)