FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18143373
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCA66350.022.786977
AACACTC72550.021.1373446
CACTCAA94200.015.973638
ACTCAAC103400.014.9005219
TAGCATG59200.014.4721658
AAACACT119300.013.0956645
CAAACAC131550.011.9856474
GTGAGTA46200.010.6131612
TACAAAC162200.09.7357872
ACAAACA183550.08.9569113
GGCCTAA36650.08.8400165-69
AGTGAGT49150.08.554931
CCTTTAG125100.07.5391474
ATACAAA250150.06.92642971
TAGGAGG53000.06.65623147
GCCCCTA60150.06.152182695-99
GAGTAGG61150.06.1223074
CTCACTA49550.05.957072715-19
AGGCCTA64450.05.920681560-64
ACTCACT56100.05.82623610-14
Produced by FastQC (version 0.11.5)