FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18143373
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCA68150.022.5020947
AACACTC77000.020.7572696
TAGCATG59250.016.5268788
CACTCAA94450.016.161228
ACTCAAC102700.015.3537319
AAACACT119600.013.1230055
CAAACAC129050.012.2181334
TACAAAC161250.010.1402712
AGTGAGT48500.09.291981
ACAAACA185300.09.092473
GTGAGTA52200.08.831472
GGCCTAA37750.08.314980565-69
CCTTTAG131350.08.1124194
ATACAAA244200.07.1734771
TTAGCAT173550.07.01085957
CTTTAGC184450.06.20620875
TACACAC65750.06.13184435-39
TTTAGCA193150.06.03848846
AGCATGA183050.06.01813559
GCCCCTA62200.05.99578695-99
Produced by FastQC (version 0.11.5)