FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17021560
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATGAACTGGGTGTAGATCTCGGT210710.12379006389543613Illumina Single End PCR Primer 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT309600.010.6959318
GATCGGA333450.010.0211811
TCGGAAG355400.09.6415913
AAGAGCG353500.09.5304157
ATCGGAA393550.08.9810692
TAGATCT77100.08.90741440-44
GAGCGTC391600.08.7135959
GGAAGAG422250.08.1321255
GAAGAGC444650.07.7386456
CGGAAGA471700.07.40169534
AGATCTC111550.06.440485540-44
TCATTAA126550.05.950143360-64
ACTGGGT121400.05.88304530-34
GGGTGTA134350.05.4989835-39
GTAGATC135150.05.487741535-39
GTGTAGA141650.05.408757735-39
CATTAAA139100.05.330500660-64
CACTATA52251.8189894E-125.23551039
CTAGATC20650.05.229046140-144
CGTATCA147500.05.22212655-59
Produced by FastQC (version 0.11.5)