FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13654604
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT23500.014.2302721
GTGAGTA29500.010.740132
TAACCCC36100.09.9203339
CCCCCTA28453.4742698E-106.6437661
TACACTA29909.731593E-106.35534958
ATAACCC61400.06.0707058
GTACACT36952.3646862E-116.03504667
GAGCACA110150.05.30814559
TCGGAAG122100.05.2487183
TATAACC85150.05.23769767
AGAGCAC107550.05.2326018
GTATAAC69250.05.19378046
GATCGGA109600.05.1737721
ACACTAT39057.5009666E-85.0533589
ATCGGAA140850.05.010122
Produced by FastQC (version 0.11.5)