FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005082501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005082501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13654604
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT23350.012.7360571
GTGAGTA29800.09.91344452
TAACCCC37500.07.48701059
TACACTA30403.0195224E-106.39368158
GTACACT35454.9112714E-116.0920837
ACACTAT39801.72804E-105.6072999
GTATAAC68950.05.53353176
GAGTATA73850.05.4607944
TGAGTAT72850.05.14043663
ATAACCC62100.05.10060748
Produced by FastQC (version 0.11.5)