FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13615461
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA39600.013.08935
CTATAGT46650.012.0366639
ACTATAG45000.011.8382848
ACACTAT46250.011.2072716
CACTATA52250.010.058027
GCCCTTA52950.08.4295894
TAGCCCT66550.07.5723782
TATTTAG66550.07.5720449
AAACACT78500.07.4284134
CCCTAAG18306.053815E-77.0810966
CAAACAC85450.06.40300463
TACCCTA29258.2945917E-106.39923674
CCCTTAT81700.06.3443855
ATCAAAC117750.05.58929441
ACCCTAA30703.3157994E-65.1589755
TCAAACA116650.05.06070952
Produced by FastQC (version 0.11.5)