FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13105441
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC99100.017.2913659
AGAGCGT105900.015.7731338
AAGAGCG128600.014.2765587
GATCGGA125750.014.0305121
GAAGAGC161450.011.2825376
TCGGAAG169950.010.67592053
ATCGGAA209950.08.9509432
GGAAGAG235500.07.79603155
TAGATCT55650.07.14177540-44
CGGAAGA275400.07.1110044
GTAGATC71250.05.901452535-39
GTAGGGA65850.05.75139915-19
CCCTAAA50350.05.43500041
TGTAGGG64400.05.34423515-19
AGATCTC83650.05.129943440-44
GTCGTGT65050.05.04711810-14
Produced by FastQC (version 0.11.5)