FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19028876
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA31650.015.0148237
ACACACT54950.09.1723446
GAGCACA141000.08.2211329
AGAGCAC141500.07.937678
ACACTAT62450.07.72444448
GATCGGA159500.07.54240941
AGTGAGT41200.07.5184021
AACACAC70500.07.2513565
CACTATA68250.06.9625169
ATCTAGC15450.06.244570725-29
CAACACA95100.06.13273954
GTGAGTA47950.06.00687742
CTAGCTA18000.05.91991525-29
TCGGAAG204200.05.7122773
TAACCCC55500.05.44855369
ATCGGAA261800.05.03336952
TCTAGCT22450.05.003046525-29
AAGAGCA249100.05.00060277
Produced by FastQC (version 0.11.5)