FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14896218
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATTGCGTACGTGTAGATCTCGGT237580.15949014709639722Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA147550.032.0176541
TCGGAAG199950.023.2246463
AGAGCGT199050.022.7845068
ATCGGAA217100.021.699022
GAGCGTC242350.019.1614769
AAGAGCG244450.018.8479567
GGAAGAG269950.017.4142285
GAAGAGC284650.016.691956
CGGAAGA322000.014.46524054
TACGTGT72600.013.2867930-34
ACGTGTA78650.012.3761535-39
TAGATCT83500.011.48508940-44
GTACGTG91900.010.71574830-34
GTAGGGA99000.010.13730115-19
TAGGGAA96600.010.07614215-19
CGTACGT98900.09.95730230-34
TGTAGGG100700.09.50860815-19
GTAGATC111300.09.0302135-39
GTGTAGA116850.08.73685535-39
TCGTGTA108700.08.46565410-14
Produced by FastQC (version 0.11.5)