FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12213241
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCAG73150.022.939014
TCTCTCA76200.022.8731233
TCTCAGC92550.018.7438355
CTCAGCC123300.014.9449596
TTCTCTC132750.014.0028852
TCAGCCT158950.012.0461387
TTTCTCT182400.010.236561
AGCCTGC202750.09.9055189
CAGCCTG222650.08.793878
ACTTGTG60650.06.59852515-19
CTACACT39750.06.592725375-79
TAAGCTT62400.06.29860530-34
TCAGAGG69400.05.80846425-29
GCCACTA44200.05.602911570-74
GTAAGCT70900.05.54348330-34
TGTGAAT77650.05.357961720-24
GTGAATC73850.05.341254720-24
CTTGTGA86900.05.085708615-19
Produced by FastQC (version 0.11.5)