FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17104859
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATA51050.013.3972159
AACACTA52500.013.0267397
ACACTAT52250.012.9511388
ACCAAAC91800.08.3959863
AAACACT113300.06.86223036
TACCAAA123050.06.67399022
CCAAACA112150.06.3589714
ATACCAA139750.06.3022321
CAAACAC128800.06.09318455
CCTTTAG48750.05.7628974
CCCTAAG20351.5170051E-45.6601376
GCCCTTA64550.05.57987744
CGTTAGG20050.05.314006315-19
CTAAGAG28906.0589955E-65.2310378
Produced by FastQC (version 0.11.5)