FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13267611
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA34750.06.83672487
GATCGGA123500.06.70230051
AGAGCGT131450.05.7505298
ATCGGAA176300.05.31000042
ACACTAT42057.385097E-105.3073158
TCGGAAG147450.05.2243963
CACTATA51007.2759576E-125.22280849
AAGAGCG160350.05.1631877
Produced by FastQC (version 0.11.5)