FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13743029
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA115300.08.1331671
AGAGCAC108000.07.9864048
GAGCACA108200.07.7014179
ACACTAC29351.8189894E-127.1149713
TCGGAAG135100.06.50261073
GTACACT33854.9112714E-106.01310251
CTACGTT40102.3646862E-115.83308949
ATCGGAA165700.05.73633772
CACTACT40253.8198777E-115.72485164
TACACTA35602.1173037E-95.66358382
ACTACGT41854.3473847E-105.41451078
AAGAGCA180050.05.27371557
Produced by FastQC (version 0.11.5)