FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11613718
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT72950.015.1007298
TAGCCCT45800.014.7909022
CACTATA75750.014.2566669
CAAACAC83700.013.3324515
AACACTA82050.013.25060757
GCCCTTA56400.011.8784614
GTAGCCC61200.011.2377051
AAACACT105100.010.6847396
AGCCCTT65450.010.5661773
CCAAACA116550.09.8892654
ACCAAAC113400.09.8463443
CCCTTAT97300.07.54729135
GATCGGA310850.06.9052734140-144
TATTTAG111900.06.8843529
CCTTATT118400.06.870196
AGATCGG336200.06.8684816140-144
GGCCCCA36900.06.67168945-49
TGAGGCC39850.06.57524240-44
CCCCTCA39650.06.53590185-89
AGGCCCC38500.06.50660745-49
Produced by FastQC (version 0.11.5)