FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19260359
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA90950.015.2798117
ACACTAT92750.015.1426748
CACTATA93450.014.9521719
CAAACAC138750.010.320495
ACCAAAC152450.09.6780253
AAACACT151000.09.5313816
TAGCCCT82000.08.9525942
CCAAACA174100.08.6397264
GCCCTTA89800.08.3350734
GTAGCCC112450.06.30816171
TATTTAG130750.06.05944549
AGCCCTT139750.05.9740173
GGCCCCT59950.05.54493585-89
AGGCCCC58000.05.338085745-49
CCCTTAT162500.05.18098975
GGCCCCA63100.05.157677745-49
TACCAAA307200.05.1073762
Produced by FastQC (version 0.11.5)