FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14864071
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTA63500.010.0892082
TAGCATG57650.09.8617988
AGTGAGT64850.09.7689321
ACATACC104400.09.3749018
AACATAC109900.08.9716057
TAAGGGG80550.08.8450029
TACGTTA42950.08.7133963
ACGTTAA43750.08.0605624
GTTACGT43850.07.55201151
CTAGGAG22800.06.17699850-54
CATACCA168000.06.16852769
CGTTAAT63600.06.11060055
TGAGTAT123100.06.0802663
GATCGGA94800.05.84731861
ATAAGGG148050.05.59007648
TTTTAGC108100.05.5258575
GAGTATA137350.05.50183964
AAACATA191650.05.482666
GTATAAG147550.05.12150246
CCACTAG25300.05.120121540-44
Produced by FastQC (version 0.11.5)