FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370950
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC34300.011.3352767
TAGACCC35550.010.5316468
GTTAGAC36250.010.5268966
GACACTA38000.010.4210539
CGACACT44650.010.1590158
AGACCCC38700.09.4883729
GTCTCAG46900.08.4434971
TAGTTAG46900.08.2899794
GCGACAC72900.06.4197537
AGTTAGA65950.06.11372235
CTCAGCG72950.05.82316643
GATCGGA159550.05.76722140-144
AGATCGG178250.05.477251140-144
TAGATCG54400.05.320588140-144
CTAGATC14700.05.191837135-139
Produced by FastQC (version 0.11.5)