FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370950
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC37650.09.7529896
TTAGACC33500.09.6716427
TAGACCC37450.08.2670238
AGACCCC40500.08.1777789
GACACTA36500.08.0876719
TAGTTAG46550.06.9602584
GTCTCAG46600.06.489271
CGACACT45700.06.3019698
GATCGGA160800.05.7492533140-144
AGTTAGA69450.05.39092875
AGATCGG179600.05.3398666140-144
TAGATCG54800.05.176642140-144
Produced by FastQC (version 0.11.5)