FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16746121
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA228100.09.2504471
AGAGCGT263500.06.9670778
TCGGAAG273150.06.9073353
GAGCGTC280300.06.5501659
GTATAGG65400.06.3854671
AAGAGCG314550.06.04353337
ATCGGAA325350.05.9104272
GTTAGAC18852.5911658E-45.83009241
GTTCCTA46700.05.8047081
GGTACGT54250.05.6721161
TAGATCT64750.05.581305540-44
GGTAGGT72400.05.4645041
GAAGAGC354900.05.43757726
GATCCAG151650.05.41092061
GGTACAA85150.05.33460861
GTGTACA83900.05.32676361
GATCATG183850.05.3000941
GTTAGTG22555.0968915E-45.19839141
GGTAGGA67000.05.13947251
GTATGTC92700.05.1372321
Produced by FastQC (version 0.11.5)