FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005083825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005083825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12151595
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA44700.027.3818829
GCCACTC47100.025.6815368
AAGCCAC89850.014.0234346
AGCCACT94850.013.4359557
ACAAAGC116200.011.0298533
CCGTTAA72600.010.711324
CGTTAAA78250.010.2134635
TCCGTTA83850.09.3600743
TACAAAG161900.08.2722172
ATACAAA161250.08.2628811
CTCACTA32950.07.82287315-19
AAAGCCA191000.07.27542455
GTTAAAT107550.07.1632116
GGCCTAA35850.07.10984775-79
GTTCCGT114900.07.08301071
GCCCCTA38250.06.6635094110-114
TACACAC40750.06.60827450-54
CAAAGCC220900.06.42135054
ACACACT42500.06.302284750-54
ACTCACT45850.06.061590715-19
Produced by FastQC (version 0.11.5)